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LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and dissimilation simultaneously

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1349-3

摘要： Abstract • Pseudomonas mendocina was first reported for aerobic nitrate removal. • It removed 90% of NO3−-N in 24 h under aerobic conditions. • This strain converted NO3−-N to bio-nitrogen (37.9%) and gaseous nitrogen (49.7%). • Inoculation of this strain increased sludge denitrification rate by 4.3 times. The problem of nitrate accumulation in aerobic tank and total nitrogen excessive discharge in effluent was very common in traditional livestock and poultry farming wastewater treatment systems owing to the lengthy process flow and low process control level. A strain LYX of aerobic bacterium was isolated from the activated sludge of a wastewater treatment system in a pig farm, which could remove nitrate effectively in aerobic tank and was identified Pseudomonas mendocina by 16S rRNA sequencing. Under the condition of nitrate as the sole nitrogen source, this strain removed over 90% of NO3−-N with an initial concentration of 110 mg/L under aerobic conditions within 48 hours. Among them, 37.9% of NO3−-N was assimilated into Bio-N, about 51.9% was reduced to gaseous nitrogen and less than 0.5% of nitrogen was replaced by NO2−-N and NH4+-N, 9.7% NO3−-N remained in the effluent at the end. At the same time, four key genes (napA, nirK, norB and nosZ) related to nitrate nitrogen removal were expressed during the denitrification process of P. mendocina LYX, in which the transcription level of the indicator genes of this aerobic denitrifying bacterium (napA) was the highest. In addition, it was found with the 15N tracer technique that inoculation of this strain on sludge increased the amount of nitrogen loss from 9.26 nmol N/(g·h) to 23.835 nmol N/(g·h). Therefore, P. medocina LYX is a potential bioagent for advanced nitrogen removal by assimilating and reducing nitrate simultaneously in aerobic tanks.

关键词： Pseudomonas mendocina     Aerobic nitrate removal     15N tracing technique     Denitrification assimilatively and disimilatively     Aerobic denitrifying genes    

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Negative weights in network time model

Zoltán A. VATTAI, Levente MÁLYUSZ

《工程管理前沿（英文）》 2022年 第9卷 第2期   页码 268-280 doi: 10.1007/s42524-020-0109-1

摘要： Time does not go backward. A negative duration, such as “time period” at first sight is difficult to interpret. Previous network techniques (CPM/PERT/PDM) did not support negative parameters and/or loops (potentially necessitating recursive calculations) in the model because of the limited computing and data storage capabilities of early computers. Monsieur Roy and John Fondahl implicitly introduced negative weights into network techniques to represent activities with fixed or estimated durations (MPM/PDM). Subsequently, the introduction of negative lead and/or lag times by software developers (IBM) apparently overcome the limitation of not allowing negative time parameters in time model. Referring to general digraph (Event on Node) representation where activities are represented by pairs of nodes and pairwise relative time restrictions are represented by weighted arrows, we can release most restraints in constructing the graph structure (incorporating the dynamic model of the inner logic of time plan), and a surprisingly flexible and handy network model can be developed that provides all the advantages of the abovementioned techniques. This paper aims to review the theoretical possibilities and technical interpretations (and use) of negative weights in network time models and discuss approximately 20 types of time-based restrictions among the activities of construction projects. We focus on pure relative time models, without considering other restrictions (such as calendar data, time-cost trade-off, resource allocation or other constraints).

关键词： graph technique     network technique     construction management     scheduling    

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Symbiotic performance, shoot biomass and water-use efficiency of three groundnut (

Sofiya K. MUHABA, Felix D. DAKORA

《农业科学与工程前沿（英文）》 2020年 第7卷 第4期   页码 455-466 doi: 10.15302/J-FASE-2020354

摘要：

Phosphorus is a key nutrient element involved in energy transfer for cellular metabolism, respiration and photosynthesis and its supply at low levels can affect legume nodulation, N fixation, and C assimilation. A two-year field study was conducted in Ethiopia in 2012 and 2013 to evaluate the effects of P supply on growth, symbiotic N nutrition, grain yield and water-use efficiency of three groundnut genotypes. Supplying P to the genotypes significantly increased their shoot biomass, symbiotic performance, grain yield, and C accumulation. There was, however, no effect on shoot δ C values in either year. Compared to the zero-P control, supplying 40 kg·ha P markedly increased shoot biomass by 77% and 66% in 2012 and 2013, respectively. In both years, groundnut grain yields were much higher at 20 and 30 kg·ha P. Phosphorus supply markedly reduced shoot δ N values and increased the %Ndfa and amount of N-fixed, indicating the direct involvement of P in promoting N fixation in nodulated groundnut. The three genotypes differed significantly in δ N, %Ndfa, N-fixed, grain yield, C concentration, and δ C. The phosphorus × genotype interaction was also significant for shoot DM, N content, N-fixed and soil N uptake.

关键词： shoot yield     N-fixed     %Ndfa     δ15N     δ13C     water-use efficiency    

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Asymmetric transfer hydrogenation of prochiral ketone catalyzed over Fe-CS/SBA-15 catalyst

XUE Ping, WU Tao

《化学科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 251-255 doi: 10.1007/s11705-007-0045-1

摘要： A heterogeneous chiral catalyst Fe(III)-CS (chitosan) complex/mesoporous molecular sieve SBA-15 (Santa Barbara Amorphous) was prepared. The asymmetric transfer hydrogenations of prochiral acetophenone and 4-methyl-2-pentanone to corresponding chiral alcohols were carried out on Fe-CS/SBA-15 at atmosphere pressure using 2-propanol as hydrogen donor. Effects of Fe content in catalyst, reaction temperature, reaction time and promoter KOH concentration on the conversion of substrates and enantioselectivity were investigated. Fe-CS/SBA-15 with 2.2% mass fraction Fe exhibits considerable enantioselectivity and catalytic activity for the asymmetric transfer hydrogenations of aromatic ketone and aliphatic ketone. Under optimal reaction conditions: KOH concentration 0.03 mol/L, reaction temperature 70ºC and reaction time 4 h, enantiomer excess (ee) of ()-1-phenylethanol and conversion of acetophenone can reach 87.4% and 27.7%, respectively. Under the above KOH concentration and reaction temperature and reaction time of 8 h, the ee of ()-4-methyl-2-pentanol and conversion 4-methyl-2-pentanone amounted to 50.2% and 25.5%, respectively.

关键词： asymmetric transfer     enantioselectivity     Fe-CS/SBA-15     aliphatic     aromatic    

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GDF15 negatively regulates chemosensitivity via TGFBR2-AKT pathway-dependent metabolism in esophageal

《医学前沿（英文）》 2023年 第17卷 第1期   页码 119-131 doi: 10.1007/s11684-022-0949-7

摘要： Treating patients with esophageal squamous cell carcinoma (ESCC) is challenging due to the high chemoresistance. Growth differentiation factor 15 (GDF15) is crucial in the development of various types of tumors and negatively related to the prognosis of ESCC patients according to our previous research. In this study, the link between GDF15 and chemotherapy resistance in ESCC was further explored. The relationship between GDF15 and the chemotherapy response was investigated through in vitro and in vivo studies. ESCC patients with high levels of GDF15 expression showed an inferior chemotherapeutic response. GDF15 improved the tolerance of ESCC cell lines to low-dose cisplatin by regulating AKT phosphorylation via TGFBR2. Through an in vivo study, we further validated that the anti-GDF15 antibody improved the tumor inhibition effect of cisplatin. Metabolomics showed that GDF15 could alter cellular metabolism and enhance the expression of UGT1A. AKT and TGFBR2 inhibition resulted in the reversal of the GDF15-induced expression of UGT1A, indicating that TGFBR2-AKT pathway-dependent metabolic pathways were involved in the resistance of ESCC cells to cisplatin. The present investigation suggests that a high level of GDF15 expression leads to ESCC chemoresistance and that GDF15 can be targeted during chemotherapy, resulting in beneficial therapeutic outcomes.

关键词： GDF15     esophageal squamous cell carcinoma     chemoresistance     cellular metabolism     TGFBR2     AKT    

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Enhanced desulfurization performance of hybrid membranes using embedded hierarchical porous SBA-15

Ye Zhang, Jian Song, Josue Quispe Mayta, Fusheng Pan, Xue Gao, Mei Li, Yimeng Song, Meidi Wang, Xingzhong Cao, Zhongyi Jiang

《化学科学与工程前沿（英文）》 2020年 第14卷 第4期   页码 661-672 doi: 10.1007/s11705-019-1830-3

摘要： The utilization of materials with a hierarchical porous structure as multi-functional additives is highly attractive in the preparation of hybrid membranes. In this study, novel hybrid membranes are designed by embedding hierarchical porous Santa Barbara Amorphous 15 (SBA-15) with a dual-pore architecture (micropores and mesopores) for pervaporation desulfurization. The SBA-15 with cylindrical mesopores provides molecular transport expressways to ensure improved permeability, while micropores on the wall have molecular sieving effects that are essential for the enhancement of permselectivity of thiophene molecules. Considering thiophene/ -octane mixture as a model system, the hybrid membrane with embedded 6 wt-% SBA-15 exhibits optimal pervaporation desulfurization performance with a permeation flux of 22.07 kg·m ·h and an enrichment factor of 6.76. Moreover, the detailed structure and properties of hybrid membranes are systematically characterized. This study demonstrates the immense potential of hierarchical porous materials as additives in membranes to simultaneously increase permeability and permselectivity.

关键词： hybrid membranes     hierarchical porous SBA-15     sieving effect     pervaporation     desulfurization    

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Sensory innervation of the anterior eye segment in rats: a retrograde tracing study

Haixia LIU MD , Zhiwang LI MD , Min YANG MD , Xiang TIAN MS , Chaoying LI PhD , Lei PEI PhD ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 352-356 doi: 10.1007/s11684-009-0065-y

摘要： This study aimed to investigate the sensory innervation of the anterior eye segment in rats by retrograde tracing with 1,1-dioleyl-3,3,3,3-tetrameth-ylindocarbocyanine, 4-chlorobenesulfonate (FAST Dil) injected into the anterior chamber. In our study, the sensory innervation of distinct elements of the anterior segment of the rat’s eye, i.e. the cornea, ciliary body, iris, and trabecular meshwork, were studied by retrograde tracing using FAST Dil as a tracer. FAST Dil was injected into the anterior chambers of the rat’s eyes. The animals were sacrificed at different time points, i.e., 2, 3, 4, and 5 days after the injection. FAST Dil localization in trigeminal ganglions was studied with a fluorescent microscope. Two days after FAST Dil injection into the anterior chambers, the cornea, the ciliary body, the iris, and the trabecular meshwork were heavily labeled. Neurons in the ipsilateral trigeminal ganglion were also consistently labeled. The number of labeled cells increased over time until 4 days after FAST Dil injection. FAST Dil-labeled neurons could be divided into two parts. Most of the Dil-labeled neurons were concentrated in a sharp, longitudinal, spindle-like stripe, located in the dorso-medial side of the trigeminal ganglion, approximately two thirds of the dorsal portion. The other part of Dil-labeled neurons scattered laterally to the stripe, but just in one third of the dorsal region. Thus, with our preliminary results, we conclude that the primary afferent sensory neurons innervating the rat’s anterior eye segments aggregate in the dorso-medial part of the ipsilateral trigeminal ganglion. It is feasible to identify them using retrograde tracing with FAST Dil anterior chamber injection.

关键词： trigeminal ganglion     retrograde tracing    

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A mini review on chemical fixation of CO 2 : Absorption and catalytic conversion into cyclic carbonates

Weili DAI, Chaktong AU, Shenglian LUO, Shuangfeng YIN,

《化学科学与工程前沿（英文）》 2010年 第4卷 第2期   页码 163-171 doi: 10.1007/s11705-009-0235-0

摘要： In this article, we present our research results on chemical fixation of CO using organobismuth compounds. We fabricated bismuth biphenoate complex, Zn-Mg-Al composite oxides, and SBA-15 or Al-SBA-15 immobilized hydroxyl ionic liquid for CO cycloaddition onto epoxides. The hypervalent bismuth compounds show good ability for association and dissociation with CO. The bismuth biphenolate complexes are catalytically effective for the cycloaddition reaction. The heterogeneous catalysts, viz. Zn-Mg-Al oxides and SBA-15 or Al-SBA-15 immobilized ionic liquid, are efficient for the synthesis of cyclic carbonate from CO and epoxide. It is found that the presence of a trace amount of water can improve the catalytic activity of the immobilized ionic liquid.

关键词： SBA-15     CO cycloaddition     Al-SBA-15 immobilized     CO     dissociation    

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Beneficial metabolic activities of inflammatory cytokine interleukin 15 in obesity and type 2 diabetes

null

《医学前沿（英文）》 2015年 第9卷 第2期   页码 139-145 doi: 10.1007/s11684-015-0377-z

摘要：

In obesity, chronic inflammation is believed to induce insulin resistance and impairs adipose tissue function. Although this view is supported by a large body of literature, it has been challenged by growing evidence that pro-inflammatory cytokines may favor insulin sensitivity through induction of energy expenditure. In this review article, interleukin 15 (IL-15) is used as a new example to explain the beneficial effects of the pro-inflammatory cytokines. IL-15 is secreted by multiple types of cells including macrophages, neutrophils and skeletal muscle cells. IL-15 expression is induced in immune cells by endotoxin and in muscle cells by physical exercise. Its transcription is induced by transcription factor NF-κB. IL-15 binds to its receptor that contains three different subunits (α, β and γ) to activate JAK/STAT, PI3K/Akt, IKK/NF-κB and JNK/AP1 pathways in cells. In the regulation of metabolism, IL-15 reduces weight gain without inhibiting food intake in rodents. IL-15 suppresses lipogenesis, stimulates brown fat function, improves insulin sensitivity through weight loss and energy expenditure. In human, circulating IL-15 is negatively associated with body weight. In the immune system, IL-15 stimulates proliferation and differentiation of T cells, NK cells, monocytes and neutrophils. In the anti-obesity effects of IL-15, T cells and NK cells are not required, but leptin receptor is required. In summary, evidence from human and rodents supports that the pro-inflammatory cytokine IL-15 may enhance energy expenditure to protect the body from obesity and type 2 diabetes. The mechanism of IL-15 action remains to be fully uncovered in the regulation of energy expenditure.

关键词： inflammation     obesity     cytokine     energy expenditure     insulin resistance    

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Oxidation-extraction desulfurization of model oil over Zr-ZSM-5/SBA-15 and kinetic study

Chuanzhu LU,Hui FU,Huipeng LI,Hua ZHAO,Tianfeng CAI

《化学科学与工程前沿（英文）》 2014年 第8卷 第2期   页码 203-211 doi: 10.1007/s11705-014-1420-3

摘要： ZSM-5/SBA-15 composite molecular sieves were synthesized using post-synthesis method and characterized by X-ray diffraction and N adsorption-desorption. The oxidative-extration desulfurization of model oil was investigated by using hydrogen peroxide as the oxidant, tetrabutyl ammonium bromide as phase transfer catalyst, dimethyl sulfoxide as extractant, and Zr-ZSM-5/SBA-15, Ag-ZSM-5/SBA-15, Ce-ZSM-5/SBA-15 as catalyst. Under the optimal conditions, the desulfurization rate decreases in the order: Zr-ZSM-5/SBA-15>Ce-ZSM-5/SBA-15>Ag-ZSM-5/SBA-15. The highest desulfurization rate is 84.53% under the catalysis of Zr-ZSM-5/SBA-15. Kinetics analysis shows that the reaction is pseudo-first-order with the activation energy of 44.23 kJ/mol.

关键词： composite molecular sieve     oxidation desulfuration     extraction     kinetic    

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Heat Integration retrofit analysis—an oil refinery case study by Retrofit Tracing Grid Diagram

Andreja NEMET, Jiří Jaromír KLEMEŠ, Petar Sabev VARBANOV, Valter MANTELLI

《化学科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 163-182 doi: 10.1007/s11705-015-1520-8

摘要： Heat Integration has been established over the last decades as a proven chemical engineering methodology. Two design implementations are often used in the industry: grassroots and retrofit. Although various methods have been developed for retrofit, it still needs more development to ensure simultaneously thermodynamic feasibility and economic viability. In this paper, a novel graphical approach has been developed to facilitate the understanding of the current situation and scope of improvement. The Retrofit Tracing Grid Diagram presents all streams and heat exchangers in temperature scale and the heat exchangers are clearly separated from each other, enabling clear visualisation of the current state. The tool incorporates the previously developed Cross-Pinch Analysis as well as path approach for retrofit. Additionally, the non-vertical heat transfer can be evaluated. The application of the developed tool has been validated on an oil refinery case study. The applicability of the tool is evident as it can reveal additional options for modification that none of the previous methods considered.

关键词： Heat Integration     retrofit     Pinch Analysis     thermodynamic approach     oil refinery    

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一种K自适应的广域网SDN控制器部署方法 Article

Peng XIAO,Zhi-yang LI,Song GUO,Heng QI,Wen-yu QU,Hai-sheng YU

《信息与电子工程前沿（英文）》 2016年 第17卷 第7期   页码 620-633 doi: 10.1631/FITEE.1500350

摘要： 软件定义网络（software-defined networking）作为一种新技术框架，正成为未来网络技术的核心。软件定义网络的核心思想就是控制平面和数据平面分离，方便管理和控制编程。虽然软件定义网络已在数据中心这样的局域网中得到了应用和部署，但在更大规模的广域网上部署依然面临着很多问题，如SDN域划分、控制器部署等问题。本文提出了一种基于谱聚类的SDN控制器部署方法，通过此方法能将较大的网络划分成小的SDN域并选择其控制器位置。通过分析模型的矩阵扰动和本征间隙，能够自动得到SDN域个数，以达到较好的划分效果和控制器部署方案。最后，为了验证算法的有效性，本文提出了一个新的实验框架，并基于广域网拓扑结构进行了实验。实验结果表明，该方法能很好的解决SDN域划分和控制器部署问题。

关键词： 软件定义网络；控制器部署；K自适应方法    

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Tracing the impact of stack configuration on interface resistances in reverse electrodialysis by in situ

《环境科学与工程前沿（英文）》 2022年 第16卷 第4期 doi: 10.1007/s11783-021-1480-9

摘要：

• RED performance and stack resistance were studied by EIS and LSV.

关键词： Reverse electrodialysis     Electrochemical impedance spectroscopy     Concentration polarization     Spacer shadow effect    

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General modeling and optimization technique for real-world earth observation satellite scheduling

《工程管理前沿（英文）》   页码 695-709 doi: 10.1007/s42524-023-0263-3

摘要： Over the last two decades, many modeling and optimization techniques have been developed for earth observation satellite (EOS) scheduling problems, but few of them show good generality to be engineered in real-world applications. This study proposes a general modeling and optimization technique for common and real-world EOS scheduling cases; it includes a decoupled framework, a general modeling method, and an easy-to-use algorithm library. In this technique, a framework that decouples the modeling, constraints, and optimization of EOS scheduling problems is built. With this framework, the EOS scheduling problems are appropriately modeled in a general manner, where the executable opportunity, another format of the well-known visible time window per EOS operation, is viewed as a selectable resource to be optimized. On this basis, 10 types of optimization algorithms, such as Tabu search and genetic algorithm, and a parallel competitive memetic algorithm, are developed. For simplified EOS scheduling problems, the proposed technique shows better performance in applicability and effectiveness than the state-of-the-art algorithms. In addition, a complicatedly constrained real-world benchmark exampled by a four-EOS Chinese commercial constellation is provided, and the technique is qualified and outperforms the in-use scheduling system by more than 50%.

关键词： earth observation satellite     scheduling     general technique     optimization algorithm     commercial constellation     real-world     benchmark    

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Experimental investigation on freeze−thaw durability of polymer concrete

《结构与土木工程前沿（英文）》 2021年 第15卷 第4期   页码 1038-1046 doi: 10.1007/s11709-021-0748-2

摘要： Assessing the durability of concrete is of prime importance to provide an adequate service life and reduce the repairing cost of structures. Freeze–thaw is one such test that indicates the ability of concrete to last a long time without a significant loss in its performance. In this study, the freeze–thaw resistance of polymer concrete containing different polymer contents was explored and compared to various conventional cement concretes. Concretes’ fresh and hardened properties were assessed for their workability, air content, and compressive strength. The mass loss, length change, dynamic modulus of elasticity, and residual compressive strength were determined for all types of concretes subjected to freeze–thaw cycles according to ASTM C666-procedure A. Results showed that polymer concrete (PC) specimens prepared with higher dosages of polymer contents possessed better freeze–thaw durability compared to other specimens. This high durability performance of PCs is mainly due to their impermeable microstructures, absence of water in their structure, and the high bond strength between aggregates and a polymer binder. It is also indicated that the performance of high-strength concrete containing air-entraining admixture is comparable with PC having optimum polymer content in terms of residual compressive strength, dynamic modulus of elasticity, mass loss, and length change.

关键词： durability test     freeze-thaw resistance     polymer concrete     residual compressive strength     ASTM C666-15    

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标题 作者 时间 类型 操作

LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and dissimilation simultaneously

Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu

期刊论文

Negative weights in network time model

Zoltán A. VATTAI, Levente MÁLYUSZ

期刊论文

Symbiotic performance, shoot biomass and water-use efficiency of three groundnut (

Sofiya K. MUHABA, Felix D. DAKORA

期刊论文

Asymmetric transfer hydrogenation of prochiral ketone catalyzed over Fe-CS/SBA-15 catalyst

XUE Ping, WU Tao

期刊论文

GDF15 negatively regulates chemosensitivity via TGFBR2-AKT pathway-dependent metabolism in esophageal

期刊论文

Enhanced desulfurization performance of hybrid membranes using embedded hierarchical porous SBA-15

Ye Zhang, Jian Song, Josue Quispe Mayta, Fusheng Pan, Xue Gao, Mei Li, Yimeng Song, Meidi Wang, Xingzhong Cao, Zhongyi Jiang

期刊论文

Sensory innervation of the anterior eye segment in rats: a retrograde tracing study

Haixia LIU MD , Zhiwang LI MD , Min YANG MD , Xiang TIAN MS , Chaoying LI PhD , Lei PEI PhD ,

期刊论文

A mini review on chemical fixation of CO 2 : Absorption and catalytic conversion into cyclic carbonates

Weili DAI, Chaktong AU, Shenglian LUO, Shuangfeng YIN,

期刊论文

Beneficial metabolic activities of inflammatory cytokine interleukin 15 in obesity and type 2 diabetes

null

期刊论文

Oxidation-extraction desulfurization of model oil over Zr-ZSM-5/SBA-15 and kinetic study

Chuanzhu LU,Hui FU,Huipeng LI,Hua ZHAO,Tianfeng CAI

期刊论文

Heat Integration retrofit analysis—an oil refinery case study by Retrofit Tracing Grid Diagram

Andreja NEMET, Jiří Jaromír KLEMEŠ, Petar Sabev VARBANOV, Valter MANTELLI

期刊论文

一种K自适应的广域网SDN控制器部署方法

Peng XIAO,Zhi-yang LI,Song GUO,Heng QI,Wen-yu QU,Hai-sheng YU

期刊论文

Tracing the impact of stack configuration on interface resistances in reverse electrodialysis by in situ

期刊论文

General modeling and optimization technique for real-world earth observation satellite scheduling

期刊论文

Experimental investigation on freeze−thaw durability of polymer concrete

期刊论文